Protocol for measuring intrahepatic triglyceride content in adults with non-alcohol fatty liver disease

Autor: Mark Ezpeleta, Kelsey Gabel, Sofia Cienfuegos, Shuhao Lin, Vasiliki Pavlou, Krista A. Varady
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: STAR Protocols, Vol 4, Iss 2, Pp 102230- (2023)
Druh dokumentu: article
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2023.102230
Popis: Summary: Here, we present a protocol for conducting magnetic resonance imaging proton density fat fraction (MRI-PDFF) to measure intrahepatic triglyceride (IHTG) content in adults with non-alcohol fatty liver disease (NAFLD). We describe steps for screening patients for NAFLD, MRI-PDFF scanning, and using MRI-PDFF data to quantify IHTG. This protocol can be repeated sequentially and used in weight loss trials. However, it is limited to patients with NAFLD as it does not assess non-alcoholic steatohepatitis or hepatic fibrosis.For complete details on the use and execution of this protocol, please refer to Ezpeleta et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Databáze: Directory of Open Access Journals