Epitachophoresis is a novel versatile total nucleic acid extraction method

Autor: Vladimira Datinska, Pantea Gheibi, Keynttisha Jefferson, Jaeyoung Yang, Sri Paladugu, Carolina Dallett, Ivona Voracova, Frantisek Foret, Yann Astier
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
Druh dokumentu: article
ISSN: 2045-2322
DOI: 10.1038/s41598-021-02214-1
Popis: Abstract Epitachophoresis is a novel next generation extraction system capable of isolating DNA and RNA simultaneously from clinically relevant samples. Here we build on the versatility of Epitachophoresis by extracting diverse nucleic acids ranging in lengths (20 nt–290 Kbp). The quality of extracted miRNA, mRNA and gDNA was assessed by downstream Next-Generation Sequencing.
Databáze: Directory of Open Access Journals
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