Single multimode fiber for in vivo light-field encoded nano-imaging
| Autor: | Wen, Zhong, Dong, Zhenyu, Pang, Chenlei, Kaminski, Clemens F., Deng, Qilin, Xu, Jinggang, Wang, Liqiang, Liu, Songguo, Tang, Jianbin, Chen, Wei, Liu, Xu, Yang, Qing |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Published online: 03 July 2023, Nature Photonics |
| Druh dokumentu: | Working Paper |
| DOI: | 10.1038/s41566-023-01240-x |
| Popis: | Super-resolution microscopy normally requiring complex and cumbersome optics is not applicable for in situ imaging through a narrow channel. Here, we demonstrate single hair-thin multimode fiber (MMF) endoscope (less than 250 ${\mu}m$) for in vivo light-field nano-imaging, which is called spatial-frequency tracking adaptive beacon light-field encoded nano-endoscopy (STABLE nano-endoscopy) that enables three-dimensional (3D) subcellular-scale imaging. Spatial-frequency tracking provides up to $10^3$ Hz disorder tracking that ensures stable imaging in long-haul MMFs (up to 200 m) under various conditions. Full-vector modulation and fluorescence emission difference are combined to enhance the imaging signal-to-noise ratio two times and to improve the resolution to sub-diffraction-limited 250 nm (${\lambda}/3NA$). STABLE nano-endoscopy and white-light endoscopy (WLE) are integrated to achieve cross-scale in vivo imaging inside the lumen. This high-resolution and robust observation in a minimally invasive manner paves the way to gain a deeper understanding of the disease mechanisms and to bridge clinical and biological sciences. Comment: 14 pages, 5 figures |
| Databáze: | arXiv |
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