Gd(III)-Gd(III) RIDME for In-Cell EPR Distance Determination

Autor:	Azarkh, Mykhailo, Bieber, Anna, Qi, Mian, Fischer, Jörg W. A., Yulikov, Maxim, Godt, Adelheid, Drescher, Malte
Rok vydání:	2019
Předmět:	Condensed Matter - Mesoscale and Nanoscale Physics Physics - Biological Physics
Druh dokumentu:	Working Paper
Popis:	In-cell distance determination by EPR reveals essential structural information about biomacromolecules under native conditions. We demonstrate that the pulsed EPR technique RIDME (relaxation induced dipolar modulation enhancement) can be utilized for such distance determination. The performance of in-cell RIDME has been assessed at Q band using stiff molecular rulers labelled with Gd(III)-PyMTA tags and microinjected into X. laevis oocytes. The overtone coefficients are determined to be the same for protonated aqueous solutions and inside cells. As compared to in-cell DEER (double electron-electron resonance, also abbreviated as PELDOR), in-cell RIDME features approximately 5 times larger modulation depth and does not show artificial broadening in the distance distributions due to the effect of pseudo-secular terms. Comment: 15 pages, 3 figures
Databáze:	arXiv
Externí odkaz:	http://arxiv.org/abs/1902.01254 Zobrazit plný text záznamu View this record from Arxiv