| Autor: |
Reisser, Matthias, Gebhardt, J. Christof M. |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
Nature Communications, 9(1), 5218 (2018) |
| Druh dokumentu: |
Working Paper |
| DOI: |
10.1038/s41467-018-07731-8 |
| Popis: |
Zygotic genome activation (ZGA), the onset of transcription after initial quiescence, is a major developmental step in many species, which occurs after ten cell divisions in Zebrafish embryos. How transcription factor-chromatin interactions evolve during early development to support ZGA is largely unknown. We established single molecule tracking in live developing Zebrafish embryos using reflected light-sheet microscopy to visualize the general transcription factor TATA-binding protein (TBP), and developed a novel data acquisition and analysis scheme to extract kinetic information during fast cell cycles. The chromatin-bound fraction of TBP increases during early development, compatible with increasing transcriptional activity. By quantifying TBP and DNA concentrations and their binding kinetics, we device a physical model of how the nuclear volume, which decreases during early development, enhances TBP-chromatin associations. Our single molecule data suggest that the shrinking nucleus is a major driving force and timer of ZGA in Zebrafish embryos. |
| Databáze: |
arXiv |
| Externí odkaz: |
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