| Autor: |
Louzon, D., Ginsburg, A., Schwenger, W., Dvir, T., Dogic, Z., Raviv, U. |
| Rok vydání: |
2016 |
| Předmět: |
|
| Druh dokumentu: |
Working Paper |
| DOI: |
10.1016/j.bpj.2017.02.038 |
| Popis: |
Bacterial mobility is powered by rotation of helical flagellar filaments driven by rotary motors. Flagellin isolated from {\it Salmonella Typhimurium} SJW1660 strain, which differs by a point mutation from the wild-type strain, assembles into straight filaments in which flagellin monomers are arranged into left-handed helix. Using small-angle X-ray scattering (SAXS) and osmotic stress methods, we investigated the high-resolution structure of SJW1660 flagellar filaments as well as intermolecular forces that govern their assembly into dense hexagonal bundle. The scattering data were fitted to high-resolution models, which took into account the atomic structure of the flagellin subunits. The analysis revealed the exact helical arrangement and the super-helical twist of the flagellin subunits within the filaments. Under osmotic stress the filaments formed $2D$ hexagonal bundles. Monte-Carlo simulations and continuum theories were used to analyze the scattering data from hexagonal arrays, revealing how bulk modulus, as well as how the deflection length depends on the applied osmotic stress. Scattering data from aligned flagellar bundles confirmed the predicated structure-factor scattering peak line-shape. Quantitative analysis of the measured equation of state of the bundles revealed the contributions of the electrostatic, hydration, and elastic interactions to the intermolecular interactions associated with bundling of straight semi-flexible flagellar filaments.}%1 {Insert Received for publication Date and in final form Date. |
| Databáze: |
arXiv |
| Externí odkaz: |
|
