Sensing of DNA conformation based on change in FRET efficiency between laser dyes

Autor:	Dey, Dibyendu, Saha, Jaba, Roy, Arpan Datta, Bhattacharjee, D., Sinha, Sangram, Paul, P. K., Chakraborty, Santanu, Hussain, Syed Arshad
Rok vydání:	2014
Předmět:	Condensed Matter - Materials Science Physics - Biological Physics
Zdroj:	Sens Act B 204 (2014) 746
Druh dokumentu:	Working Paper
DOI:	10.1016/j.snb.2014.08.029
Popis:	This communication reports the effect of DNA conformation on fluorescence resonance energy transfer (FRET) efficiency between two laser dyes in layer by layer (LbL) self assembled film. The dyes Acraflavine and Rhodamine B were attached onto the negative phosphate backbones of DNA in LbL film through electrostatic attraction. Then FRET between these dyes was investigated. Increase in pH or temperature causes the denaturation of DNA followed by coil formation of single stranded DNA. As a result the FRET efficiency also changed along with it. These observations demonstrated that by observing the change in FRET efficiency between two laser dyes in presence of DNA it is possible to detect the altered DNA conformation in the changed environment. Comment: 28 pages, 5 figures
Databáze:	arXiv
Externí odkaz:	http://arxiv.org/abs/1409.5097 Zobrazit plný text záznamu View this record from Arxiv