In vitro Plant Regeneration Efficiency from Different Explants of Local Sainfoin Ecotype (Onobrychis sativa)
| Autor: | TURGUT, Mehmet Burak, OĞUZ, Muhammet Çağrı, ÖNOL, Burak, SANCAK, Cengiz |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Volume: 8, Issue: 2 101-107 Ekin Journal of Crop Breeding and Genetics |
| ISSN: | 2149-1275 2459-069X |
| Popis: | Local populations and ecotypes are important genetic resources with genetic diversity. It is possible to protect threatenedgenotypes and increase biodiversity with in vitro tissue culture techniques. Explant type is of great importance among thefactors affecting the success of in vitro regeneration and callus induction. In this study, Sainfoin seeds belonging to the Gözlüecotype were used as experiment material. MS media were prepared with TDZ and combinations of IBA-NAA. Callusinduction and shoot regeneration rates of hypocotyl, leaf and cotyledon explants were determined in the sainfoin ecotype.According to the results of the study, the best callus formation and shoot regeneration were obtained from the hypocotylexplant in an MS medium containing 2 mg/L TDZ. In the present study, important results were obtained that will contributeto the regeneration studies of local ecotypes and wild species, genetic transformation and increasing genetic diversity. |
| Databáze: | OpenAIRE |
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