| Autor: |
Tafin UF, Corvec S, Betrisey B, Zimmerli W, Trampuz A |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Antimicrobial Agents and Chemotherapy |
| Popis: |
Propionibacterium acnes is an important cause of orthopedic implant associated infections for which the optimal treatment has not yet been determined. We investigated the activity of rifampin alone and in combination against planktonic and biofilm P. acnes in vitro and in a foreign body infection model. The MIC and the minimal bactericidal concentration (MBC) were 0.007 and 4 µg/ml for rifampin 1 and 4 µg/ml for daptomycin 1 and 8 µg/ml for vancomycin 1 and 2 µg/ml for levofloxacin 0.03 and 16 µg/ml for penicillin G 0.125 and 512 µg/ml for clindamycin and 0.25 and 32 µg/ml for ceftriaxone respectively. The P. acnes minimal biofilm eradication concentration (MBEC) was 16 µg/ml for rifampin 64 µg/ml for penicillin G daptomycin and ceftriaxone and =128 µg/ml for levofloxacin vancomycin and clindamycin. In the animal model implants were infected by injection of 109 CFU P. acnes in cage. Antimicrobial activity on P. acnes was investigated in the cage fluid (planktonic form) and on explanted cages (biofilm form). The cure rates were 4 for daptomycin 8 for vancomycin 0 for levofloxacin and 36 for rifampin. Rifampin cured 63 of the infected cages in combination with daptomycin 46 with vancomycin and 25 with levofloxacin. While all tested antimicrobials showed good activity against planktonic P. acnes for eradication of biofilms rifampin was needed. In combination with rifampin daptomycin showed higher cure rates than with vancomycin in this foreign body infection model. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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