Isolation of Neural Stem and Progenitor Cells from the Adult Brain and Live Imaging of Their Cell Cycle with the FUCCI System
| Autor: | Chicheportiche, Alexandra, Ruat, Martial, Boussin, François D, Daynac, Mathieu |
|---|---|
| Přispěvatelé: | Laboratoire de Radiopathologie (LRP), Université Paris Diderot - Paris 7 (UPD7)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut des Neurosciences Paris-Saclay (NeuroPSI), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Department of Medicine [San Francisco], University of California [San Francisco] (UCSF), University of California-University of California, Institut des Neurosciences de Paris-Saclay (Neuro-PSI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), University of California [San Francisco] (UC San Francisco), University of California (UC)-University of California (UC) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
FUCCI
Neural stem and progenitor cells [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology FACS Brain [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences Mice Transgenic Cell Separation Cell cycle Flow Cytometry Luminescent Proteins Mice Microscopy Fluorescence Neural Stem Cells nervous system Sub-ventricular zone Animals Time-lapse video microscopy Cells Cultured reproductive and urinary physiology Cell Proliferation |
| Zdroj: | Methods in Molecular Biology Methods in Molecular Biology, Humana Press/Springer Imprint, 2018, Cellular Quiescence, 1686, pp.69-78. ⟨10.1007/978-1-4939-7371-2_5⟩ Methods in Molecular Biology, 2018, Cellular Quiescence, 1686, pp.69-78. ⟨10.1007/978-1-4939-7371-2_5⟩ |
| ISSN: | 1064-3745 1940-6029 |
| DOI: | 10.1007/978-1-4939-7371-2_5⟩ |
| Popis: | International audience; Neural stem cells (NSCs) enter quiescence in early embryonic stages to create a reservoir of dormant NSCs able to enter proliferation and produce neuronal precursors in the adult mammalian brain. Various approaches of fluorescent-activated cell sorting (FACS) have emerged to allow the distinction between quiescent NSCs (qNSCs), their activated counterpart (aNSCs), and the resulting progeny. In this article, we review two FACS techniques that can be used alternatively. We also show that their association with transgenic Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) mice allows an unprecedented overlook on the cell cycle dynamics of adult NSCs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|