'Don, doff, discard' to 'don, doff, decontaminate'-FFR and mask integrity and inactivation of a SARS-CoV-2 surrogate and a norovirus following multiple vaporised hydrogen peroxide-, ultraviolet germicidal irradiation-, and dry heat decontaminations

Autor: Ludwig-Begall, Louisa F., Wielick, Constance, Jolois, Olivier, Dams, Lorene, Razafimahefa, Ravo M., Nauwynck, Hans, Demeuldre, Pierre-Francois, Napp, Aurore, Laperre, Jan, Thiry, Etienne, Haubruge, Eric
Jazyk: angličtina
Rok vydání: 2021
Předmět:
RNA viruses
Hot Temperature
Pulmonology
Sanitization
Coronaviruses
PORCINE RESPIRATORY CORONAVIRUS
Respirators
Medical Conditions
Anti-Infective Agents
Medicine and Health Sciences
Public and Occupational Health
Respiratory Protective Devices
Decontamination
Pathology and laboratory medicine
Virus Testing
Masks
Oxides
Medical microbiology
Peroxides
Multidisciplinary Sciences
Chemistry
Infectious Diseases
Physical Sciences
Viruses
TRANSMISSIBLE GASTROENTERITIS
Engineering and Technology
Science & Technology - Other Topics
Medicine
Ultraviolet Therapy
Safety Equipment
Safety
SARS CoV 2
Pathogens
Research Article
Biotechnology
Infectious Disease Control
SARS coronavirus
Ultraviolet Rays
Science
Equipment
Bioengineering
Microbiology
Caliciviruses
Respiratory Disorders
Diagnostic Medicine
Equipment Reuse
Humans
Veterinary Sciences
Pandemics
Personal Protective Equipment
Ventilators
Mechanical
Science & Technology
SARS-CoV-2
Norovirus
Chemical Compounds
Organisms
Viral pathogens
COVID-19
Biology and Life Sciences
Hydrogen Peroxide
EFFICACY
Microbial pathogens
Health Care
Respiratory Infections
Medical Devices and Equipment
Preventive Medicine
Volatilization
Zdroj: PLoS ONE, Vol 16, Iss 5, p e0251872 (2021)
PLoS ONE
PLOS ONE
ISSN: 1932-6203
Popis: BACKGROUND: As the SARS-CoV-2 pandemic accelerates, the supply of personal protective equipment remains under strain. To combat shortages, re-use of surgical masks and filtering facepiece respirators has been recommended. Prior decontamination is paramount to the re-use of these typically single-use only items and, without compromising their integrity, must guarantee inactivation of SARS-CoV-2 and other contaminating pathogens. AIM: We provide information on the effect of time-dependent passive decontamination (infectivity loss over time during room temperature storage in a breathable bag) and evaluate inactivation of a SARS-CoV-2 surrogate and a non-enveloped model virus as well as mask and respirator integrity following active multiple-cycle vaporised hydrogen peroxide (VHP), ultraviolet germicidal irradiation (UVGI), and dry heat (DH) decontamination. METHODS: Masks and respirators, inoculated with infectious porcine respiratory coronavirus or murine norovirus, were submitted to passive decontamination or single or multiple active decontamination cycles; viruses were recovered from sample materials and viral titres were measured via TCID50 assay. In parallel, filtration efficiency tests and breathability tests were performed according to EN standard 14683 and NIOSH regulations. RESULTS AND DISCUSSION: Infectious porcine respiratory coronavirus and murine norovirus remained detectable on masks and respirators up to five and seven days of passive decontamination. Single and multiple cycles of VHP-, UVGI-, and DH were shown to not adversely affect bacterial filtration efficiency of masks. Single- and multiple UVGI did not adversely affect respirator filtration efficiency, while VHP and DH induced a decrease in filtration efficiency after one or three decontamination cycles. Multiple cycles of VHP-, UVGI-, and DH slightly decreased airflow resistance of masks but did not adversely affect respirator breathability. VHP and UVGI efficiently inactivated both viruses after five, DH after three, decontamination cycles, permitting demonstration of a loss of infectivity by more than three orders of magnitude. This multi-disciplinal approach provides important information on how often a given PPE item may be safely reused. ispartof: PLOS ONE vol:16 issue:5 ispartof: location:United States status: published
Databáze: OpenAIRE
Externí odkaz: