Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy

Autor: de Medeiros, Gustavo, Kromm, Dimitri, Balazs, Balint, Norlin, Nils, Günther, Stefan, Izquierdo, Emiliano, Ronchi, Paolo, Komoto, Shinya, Krzic, Uros, Schwab, Yannick, Peri, Francesca, de Renzis, Stefano, Leptin, Maria, Rauzi, Matteo, Hufnagel, Lars
Přispěvatelé: Bodescot, Myriam, Centres d'excellences - Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie - - SIGNALIFE2011 - ANR-11-LABX-0028 - LABX - VALID, Etude sur les Propriétés Emergeantes à l'Echelle de l'Embryon Dirigeantes la Morphogène des Tissus - - SEPASEDTM2016 - ANR-16-TERC-0018 - TERC - VALID, European Molecular Biology Laboratory [Heidelberg] (EMBL), Friedrich Miescher Institute for Biomedical Research [Bâle, Suisse] (FMI), University of Basel (Unibas)-Novartis Institutes for BioMedical Research (NIBR), Faculty of Biosciences [Heidelberg, Allemagne], Heidelberg University, Luxendo GmbH [Heidelberg, Allemagne], Imaging Section [Onna, Okinawa, Japon], Okinawa Institute of Science and Technology Graduate University (OIST), Institute of Molecular Life Sciences [Zurich, Suisse] (IMLS), Universität Zürich [Zürich] = University of Zurich (UZH), Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), This work was supported by the European Molecular Biology Laboratory and the EMBL International PhD Programme (G.M., D.K., B.B.). We thank E. Mottay and the laser company Amplitude Systemes for their continuous support and collaboration. We thank TOPTICA Photonics AG for their support. M.L. thanks EMBO and EMBL for support. G.M. acknowledges support from the EMBO ALTF 571-2018. N.N. acknowledges support from Vinnova, Åke Wiberg foundation and Magnus Bergvall foundation. M.R. acknowledges support from the French government through the UCAJEDI Investments in the Future project managed by the National Research Agency (ANR-15 IDEX-01), the 'Investments for the Future' LABEX SIGNALIFE (ANR-11-LABX-0028-01), the Tramplin ERC program from the National Research Agency (ANR-16-TERC-0018-01), the ATIP-Avenir grant from the CNRS and the Human Frontier Science Program (CDA00027/2017-C)., ANR-11-LABX-0028,SIGNALIFE,Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie(2011), ANR-16-TERC-0018,SEPASEDTM,Etude sur les Propriétés Emergeantes à l'Echelle de l'Embryon Dirigeantes la Morphogène des Tissus(2016), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), ANR-15-IDEX-0001,UCA JEDI,Idex UCA JEDI (2016), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Novartis Institutes for BioMedical Research (NIBR)-University of Basel (Unibas), University of Zurich, Rauzi, Matteo
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Scientific Reports
Scientific Reports, 2020, 10 (1), pp.1942. ⟨10.1038/s41598-019-54349-x⟩
Scientific Reports, Nature Publishing Group, 2020, 10 (1), ⟨10.1038/s41598-019-54349-x⟩
Scientific Reports, Nature Publishing Group, 2020, 10 (1), pp.1942. ⟨10.1038/s41598-019-54349-x⟩
Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
ISSN: 2045-2322
Popis: International audience; Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.
Databáze: OpenAIRE
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