PtaRHE1, a Populus tremula × Populus alba RING-H2 protein of the ATL family, has a regulatory role in secondary phloem fibre development

Autor: Baldacci-Cresp, Fabien, Moussawi, Jihad, Leplé, Jean-Charles, Van den ackerveken, Rebecca, Kohler, Annegret, Candiracci, Julie, Twyffels, Laure, Spokevicius, Antanas V., Bossinger, Gerd, Laurans, Françoise, Brunel-Michac, Nicole, Vermeersch, Marjorie, Boerjan, Wout, El Jaziri, Mondher, Baucher, Marie
Přispěvatelé: Laboratoire de Biotechnologie Végétale, Université Libre de Bruxelles [Bruxelles] (ULB), Unité de recherche Amélioration, Génétique et Physiologie Forestières (UAGPF), Institut National de la Recherche Agronomique (INRA), Interactions Arbres-Microorganismes (IAM), Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA), Laboratoire de Biochnologie Végétale, Center for Microscopy and Molecular Imaging-CMMI, School of Ecosystem and Forest Sciences, University of Melbourne, Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier (PIAF), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Center for Microscopy and Molecular Imaging (IBMM - CMMI), Dept of Plant Systems Biology and Dept of Plant Biotechnology and Bioinformatics, Ghent University [Belgium] (UGENT), Université libre de Bruxelles (ULB), Unité de recherche Amélioration, Génétique et Physiologie Forestières (AGPF), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Universiteit Gent = Ghent University [Belgium] (UGENT), Universiteit Gent = Ghent University (UGENT)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: Plant Journal
Plant Journal, Wiley, 2015, 82 (6), pp.978-990. ⟨10.1111/tpj.12867⟩
Plant Journal, 2015, 82 (6), pp.978-990. ⟨10.1111/tpj.12867⟩
ISSN: 0960-7412
1365-313X
Popis: REALLY INTERESTING NEW GENE (RING) proteins play important roles in the regulation of many processes by recognizing target proteins for ubiquitination. Previously, we have shown that the expression of PtaRHE1, encoding a Populus tremula × Populus alba RING-H2 protein with E3 ubiquitin ligase activity, is associated with tissues undergoing secondary growth. To further elucidate the role of PtaRHE1 in vascular tissues, we have undertaken a reverse genetic analysis in poplar. Within stem secondary vascular tissues, PtaRHE1 and its corresponding protein are expressed predominantly in the phloem. The downregulation of PtaRHE1 in poplar by artificial miRNA triggers alterations in phloem fibre patterning, characterized by an increased portion of secondary phloem fibres that have a reduced cell wall thickness and a change in lignin composition, with lower levels of syringyl units as compared with wild-type plants. Following an RNA-seq analysis, a biological network involving hormone stress signalling, as well as developmental processes, could be delineated. Several candidate genes possibly associated with the altered phloem fibre phenotype observed in amiRPtaRHE1 poplar were identified. Altogether, our data suggest a regulatory role for PtaRHE1 in secondary phloem fibre development.
Databáze: OpenAIRE
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