Lectin binding patterns and immunohistochemical antigen detection in placenta and lungs of brucella abortus-bovine infected fetuses
| Autor: | Fiorentino, María Andrea, Paolicchi, Fernando Alberto, Campero, Carlos Manuel, Barbeito, Claudio G. |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Bovine fetuses
Short Communication Placenta BRUCELLA ABORTUS Brucella abortus Lectinas Feto Lectins Bovina Immunohistochemical Infección PLACENTA Antigens Immunological Techniques BOVINE FETUSES Ciencias Veterinarias Foetus Antígenos LECTIN QL1-991 CIENCIAS AGRÍCOLAS embryonic structures Ganado Bovino Técnicas Inmunológicas Cattle Pulmones Lungs purl.org/becyt/ford/4.3 [https] Infection IMMUNOHISTOCHEMICAL Zoology Lectin purl.org/becyt/ford/4 [https] Bovine fetuses Brucella abortus Immunohistochemical Lectin Placenta |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Open veterinary journal 8 (1), 57-63. (2018) INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA Open Veterinary Journal; Vol 8, No 1 (2018); 57-63 Open Veterinary Journal, Vol 8, Iss 1, Pp 57-63 (2018) Open Veterinary Journal |
| ISSN: | 2218-6050 2226-4485 |
| Popis: | Lectin binding relies on the affinity of these substances for specific terminal sugars. The method facilitates the identification of complex structures to which the terminal sugar attaches and may reveal physiological or pathological changes in cells, intracellular interactions or extracellular transport pathways. This study was carried out to investigate the effect of infection with Brucella abortus on the pattern of lectin binding in bovine fetal lungs (n=6) and bovine placentas (n=5). Fetal lungs and placenta from heifers experimentally inoculated with B. abortus, strain 2308 were examined by histological, lectin-histochemical, immunohistochemical and cultural techniques. B. abortus antigens were immunohistochemically detected in fetal lungs and placenta. An increase in the labeling with UEA-1, DBA, PNA, RCA-1 and SBA was found in the lungs and an increase in the labeling with UEA-1, ConA, PNA, DBA was found in the placentas. The present lectin histochemical study revealed a distinctive pattern of oligosaccharide distribution in the lungs and placenta of B. abortus-infected fetuses. Facultad de Ciencias Veterinarias |
| Databáze: | OpenAIRE |
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