Fungal treatment for the valorization of technical soda lignin
| Autor: | Daou, Mariane, Farfan Soto, Clementina, Majira, Amel, Cézard, Laurent, Cottyn, Betty, Pion, Florian, Navarro, David, Correia, Lydia Oliveira, Drula, Elodie, Record, Eric, Raouche, Sana, Baumberger, Stéphanie, Faulds, Craig B. |
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| Přispěvatelé: | Biodiversité et Biotechnologie Fongiques (BBF), Aix Marseille Université (AMU)-École Centrale de Marseille (ECM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Bio Based Industry Joint Undertaking under the European Union 720303LabEx Saclay Plant Sciences-SPS ANR-10-LABX-552 0040-SPS, ANR-10-LABX-0040,SPS,Saclay Plant Sciences(2010), European Project: 9720303(1998), AgroParisTech |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Leiotrametes menziesii
[SDV.BIO]Life Sciences [q-bio]/Biotechnology fungi filamentous fungi technology industry and agriculture food and beverages Pycnoporus sanguineus macromolecular substances Polyporus brumalis complex mixtures Article oxidoreductases lcsh:Biology (General) technical lignin secretomic analysis [CHIM]Chemical Sciences lcsh:QH301-705.5 |
| Zdroj: | Journal of Fungi Journal of Fungi, 2021, 7 (1), pp.39. ⟨10.3390/jof7010039⟩ Journal of Fungi, MDPI, 2021, 7 (1), pp.39. ⟨10.3390/jof7010039⟩ Journal of Fungi, Vol 7, Iss 39, p 39 (2021) Volume 7 Issue 1 |
| ISSN: | 2309-608X |
| DOI: | 10.3390/jof7010039⟩ |
| Popis: | Technical lignins produced as a by-product in biorefinery processes represent a potential source of renewable carbon. In consideration of the possibilities of the industrial transformation of this substrate into various valuable bio-based molecules, the biological deconstruction of a technical soda lignin by filamentous fungi was investigated. The ability of three basidiomycetes (Polyporus brumalis,Pycnoporus sanguineusandLeiotrametes menziesii) to modify this material, the resultant structural and chemical changes, and the secreted proteins during growth on this substrate were investigated. The three fungi could grow on the technical lignin alone, and the growth rate increased when the media were supplemented with glucose or maltose. The proteomic analysis of the culture supernatants after three days of growth revealed the secretion of numerous Carbohydrate-Active Enzymes (CAZymes). The secretomic profiles varied widely between the strains and the presence of technical lignin alone triggered the early secretion of many lignin-acting oxidoreductases. The secretomes were notably rich in glycoside hydrolases and H2O2-producing auxiliary activity enzymes with copper radical oxidases being induced on lignin for all strains. The lignin treatment by fungi modified both the soluble and insoluble lignin fractions. A significant decrease in the amount of soluble higher molar mass compounds was observed in the case ofP. sanguineus. This strain was also responsible for the modification of the lower molar mass compounds of the lignin insoluble fraction and a 40% decrease in the thioacidolysis yield. The similarity in the activities ofP. sanguineusandP. brumalisin modifying the functional groups of the technical lignin were observed, the results suggest that the lignin has undergone structural changes, or at least changes in its composition, and pave the route for the utilization of filamentous fungi to functionalize technical lignins and produce the enzymes of interest for biorefinery applications. |
| Databáze: | OpenAIRE |
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