Anaerobic sulfatase-maturating enzymes: radical SAM enzymes able to catalyze in vitro sulfatase post-translational modification

Autor: Bucharles, Christine, Bizet, Patrice, Arthaud, Sébastien, Arabo, Arnaud, Leprince, Jérôme, Lefranc, Benjamin, Cartier, Dorthe, Anouar, Youssef, Lihrmann, Isabelle
Přispěvatelé: Neuroendocrinologie cellulaire et moléculaire, Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie (PRIMACEN), Normandie Université (NU)-Normandie Université (NU)-Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Psychologie et Neurosciences de la Cognition et de l'affectivité (PSY-NCA), Normandie Université (NU)-Normandie Université (NU), Différenciation et communication neuronale et neuroendocrine (DC2N)
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Male
MESH: Signal Transduction
PPARγ
Peptide Hormones
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
MESH: Receptors
G-Protein-Coupled
[CHIM.THER]Chemical Sciences/Medicinal Chemistry
MESH: Protein Isoforms
MESH: Thapsigargin
autoradiography
Iodine Radioisotopes
MESH: Spinal Cord
antimicrobial peptides
MESH: Protein Structure
Tertiary
MESH: Autoradiography
Candida albicans
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
MESH: Glial Fibrillary Acidic Protein
MESH: Animals
c-Fos
MESH: Proto-Oncogene Proteins c-fos
MESH: Iodine Radioisotopes
UT receptor
MESH: Fourth Ventricle
dextran
immunohistochemistry
Proto-Oncogene Proteins c-fos
MESH: Ventromedial Hypothalamic Nucleus
MESH: Rats
Urotensins
brain
CSF
MESH: Radioimmunoassay
MESH: Brain
inflammasome
Glial Fibrillary Acidic Protein
host defense peptide
Animals
Vimentin
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Rats
Wistar
ventricular system
Fourth Ventricle
Binding Sites
MESH: Urotensins
Macrophages
neuropeptides
MESH: Immunohistochemistry
MESH: Rats
Wistar
MESH: Male
arachidonic acid metabolism
MESH: Binding Sites
drug delivery
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
MESH: Peptide Hormones
nanoparticles
MESH: Vimentin
C-type lectin receptors
Zdroj: Journal of Comparative Neurology
Journal of Comparative Neurology, John Wiley & Sons, 2014, 522 (11), pp.2634-2649. ⟨10.1002/cne.23553⟩
ISSN: 0092-7317
1550-7130
DOI: 10.1002/cne.23553⟩
Popis: International audience; Urotensin II (UII) and Urotensin II-related peptide (URP) are structurally related paralog peptides that exert peripheral and central effects. UII binding sites have been partly described in brain, and those of URP have never been reported. We exhaustively compared [(125)I]-UII and -URP binding site distributions in the adult rat brain, and found that they fully overlapped at the regional level. We observed UII/URP binding sites in structures lining ventricles, comprising the sphenoid nucleus and cell rafts scattered on a line joining the fourth ventricle and its lateral recess. After injection of UII and URP in the lateral ventricle, we observed c-Fos-positive cell nuclei in areas close to the fourth ventricle, indicating that these receptors are functional. Different c-Fos-containing cell populations were activated. They were all positive for vimentin and glial fibrillary acidic protein (GFAP), excluding the possibility of an ependymal nature. In conclusion, this study demonstrated that UII and URP binding sites are totally overlapping and that these sites were functional in regions bordering the fourth ventricle. These data support a role for UII/URP at the interface between brain parenchyma and cerebrospinal fluid.; Octylglyceryl dextran-graft-poly(lactic acid) nanoparticles formulated by emulsification demonstrate potential for peptide delivery.
Databáze: OpenAIRE
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