Improving the antiprotozoal effect of saponins in the rumen by combination with glycosidase inhibiting iminosugars or by modification of their chemical structure
| Autor: | Ramos-Morales, Eva, de la Fuente, Gabriel, Nash, Robert J., Braganca, Radek, Duval, Stephane, Bouillon, Marc E., Lahmann, Martina, Newbold, C. Jamie |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Metabolic Processes
Pathogen Motility Rumen Glycoside Hydrolases Virulence Factors Antiprotozoal Agents lcsh:Medicine Pathology and Laboratory Medicine Biochemistry complex mixtures Drug Stability Ammonia parasitic diseases Medicine and Health Sciences Animals lcsh:Science Nuclear Physics Protozoans Mammals Radiochemistry Ethanol Bacteria Organic Compounds Plant Extracts Physics Microbiota Organic Chemistry lcsh:R Organisms Chemical Compounds Biology and Life Sciences Ruminants Saponins musculoskeletal system carbohydrates (lipids) Chemistry Metabolism Radioactivity Alcohols Fermentation Physical Sciences Vertebrates Amniotes lcsh:Q Propionates Pathogens Research Article |
| Zdroj: | Repositorio Abierto de la UdL Universitad de Lleida PLoS ONE, Vol 12, Iss 9, p e0184517 (2017) Recercat. Dipósit de la Recerca de Catalunya instname PLoS ONE |
| Popis: | The antiprotozoal effect of saponins is transitory, as when saponins are deglycosylated to sapogenins by rumen microorganisms they become inactive. We hypothesised that the combination of saponins with glycosidase-inhibiting iminosugars might potentially increase the effectiveness of saponins over time by preventing their deglycosylation in the rumen. Alternatively, modifying the structure of the saponins by substituting the sugar moiety with other small polar residues might maintain their activity as the sugar substitute would not be enzymatically cleaved. The aim of this in vitro study was to evaluate the acute antiprotozoal effect and the stability of this effect over a 24 h incubation period using ivy saponins, a stevia extract rich in iminosugars, ivy saponins with stevia extract, and a chemically modified ivy saponin, hederagenin bis-succinate (HBS). The effects on fermentation parameters and rumen bacterial communities were also studied. Ivy saponins with stevia and HBS had a greater antiprotozoal effect than ivy saponins, and this effect was maintained after 24 h of incubation (P |
| Databáze: | OpenAIRE |
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