The cellular chloride channels CLIC1 and CLIC4 contribute to virus-mediated cell motility
| Autor: | Stakaityte, G, Nwogu, N, Lippiat, JD, Blair, GE, Poterlowicz, K, Boyne, JR, Macdonald, A, Mankouri, J, Whitehouse, A |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
chloride channel
viral protein Polyomavirus Infections Skin Neoplasms cell migration Proteome Incidence cell motility Microbiology Carcinoma Merkel Cell Tumor Virus Infections HEK293 Cells Chlorides Merkel cell polyomavirus Cell Movement Chloride Channels Humans channel activation Neoplasm Invasiveness Antigens Viral Tumor Cell Proliferation |
| Zdroj: | The Journal of Biological Chemistry |
| ISSN: | 1083-351X 0021-9258 |
| Popis: | Ion channels regulate many aspects of cell physiology, including cell proliferation, motility, and migration, and aberrant expression and activity of ion channels is associated with various stages of tumor development, with K⁺ and Cl⁻ channels now being considered the most active during tumorigenesis. Accordingly, emerging in vitro and preclinical studies have revealed that pharmacological manipulation of ion channel activity offers protection against several cancers. Merkel cell polyomavirus (MCPyV) is a major cause of Merkel cell carcinoma (MCC), primarily because of the expression of two early regulatory proteins termed small and large tumor antigens (ST and LT, respectively). Several molecular mechanisms have been attributed to MCPyV-mediated cancer formation but, thus far, no studies have investigated any potential link to cellular ion channels. Here we demonstrate that Cl⁻ channel modulation can reduce MCPyV ST-induced cell motility and invasiveness. Proteomic analysis revealed that MCPyV ST up-regulates two Cl⁻ channels, CLIC1 and CLIC4, which when silenced, inhibit MCPyV ST-induced motility and invasiveness, implicating their function as critical to MCPyV-induced metastatic processes. Consistent with these data, we confirmed that CLIC1 and CLIC4 are up-regulated in primary MCPyV-positive MCC patient samples. We therefore, for the first time, implicate cellular ion channels as a key host cell factor contributing to virus-mediated cellular transformation. Given the intense interest in ion channel modulating drugs for human disease. This highlights CLIC1 and CLIC4 activity as potential targets for MCPyV-induced MCC. |
| Databáze: | OpenAIRE |
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