alpha-Tanycytes of the adult hypothalamic third ventricle include distinct populations of FGF-responsive neural progenitors
| Autor: | Robins, S.C., Stewart, I., McNay, D.E., Taylor, V., Giachino, C., Götz, M., Ninkovic, J., Briancon, N., Maratos-Flier, E., Flier, J.S., Kokoeva, M.V., Placzek, M. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Aging
Epidermal Growth Factor Integrases Ependymoglial Cells Hypothalamus Immunohistochemistry Excitatory Amino Acid Transporter 1 Fibroblast Growth Factors Mice Inbred C57BL Mice Neural Stem Cells Spheroids Cellular Glial Fibrillary Acidic Protein Animals Fibroblast Growth Factor 10 Neuroglia Cell Proliferation Signal Transduction Third Ventricle |
| Zdroj: | Nat. Commun. 4:2049 (2013) |
| ISSN: | 2041-1723 |
| Popis: | Emerging evidence suggests that new cells, including neurons, can be generated within the adult hypothalamus, suggesting the existence of a local neural stem/progenitor cell niche. Here, we identify α-tanycytes as key components of a hypothalamic niche in the adult mouse. Long-term lineage tracing in vivo using a GLAST::CreER(T2) conditional driver indicates that α-tanycytes are self-renewing cells that constitutively give rise to new tanycytes, astrocytes and sparse numbers of neurons. In vitro studies demonstrate that α-tanycytes, but not β-tanycytes or parenchymal cells, are neurospherogenic. Distinct subpopulations of α-tanycytes exist, amongst which only GFAP-positive dorsal α2-tanycytes possess stem-like neurospherogenic activity. Fgf-10 and Fgf-18 are expressed specifically within ventral tanycyte subpopulations; α-tanycytes require fibroblast growth factor signalling to maintain their proliferation ex vivo and elevated fibroblast growth factor levels lead to enhanced proliferation of α-tanycytes in vivo. Our results suggest that α-tanycytes form the critical component of a hypothalamic stem cell niche, and that local fibroblast growth factor signalling governs their proliferation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|