Quantitative trait loci for resistance to Flavobacterium psychrophilum in rainbow trout: effect of the mode of infection and evidence of epistatic interactions

Autor: Fraslin, Clémence, Dechamp, Nicolas, Bernard, Maria, Krieg, Francine, Hervet, Caroline, Guyomard, René, Esquerré, Diane, Barbieri, Johanna, Kuchly, Claire, Duchaud, Éric, Boudinot, Pierre, Rochat, Tatiana, Bernardet, Jean-François, Quillet, Edwige
Přispěvatelé: Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut National Polytechnique de Toulouse - INPT (FRANCE), Institut National de la Recherche Agronomique - INRA (FRANCE), Université Paris-Saclay (FRANCE), French Government BPI France, Bretagne regional authority, Languedoc-Roussillon regional authority, Aquitaine regional authority, Landes regional council, Pyrenees Atlantiques regional council, France Genomique national infrastructure as part of ' Investissement d'avenir' program ANR-10-INBS-09, CIFRE scholarship 2015/0778, European Project: 262336,EC:FP7:INFRA,FP7-INFRASTRUCTURES-2010-1,AQUAEXCEL(2011), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Apollo - University of Cambridge Repository
Rok vydání: 2018
Předmět:
Zdroj: Genetics, Selection, Evolution : GSE
Genetics Selection Evolution
Genetics Selection Evolution, BioMed Central, 2018, 50 (1), pp.60. ⟨10.1186/s12711-018-0431-9⟩
Genetics Selection Evolution, Vol 50, Iss 1, Pp 1-16 (2018)
Fraslin, C, Dechamp, N, Bernard, M, Krieg, F, Hervet, C, Guyomard, R, Esquerré, D, Barbieri, J, Kuchly, C, Duchaud, E, Boudinot, P, Rochat, T, Bernardet, JF & Quillet, E 2018, ' Quantitative trait loci for resistance to Flavobacterium psychrophilum in rainbow trout: effect of the mode of infection and evidence of epistatic interactions. ', Genetics Selection Evolution . https://doi.org/10.1186/s12711-018-0431-9
Genetics Selection Evolution, 2018, 50 (1), pp.60. ⟨10.1186/s12711-018-0431-9⟩
ISSN: 1297-9686
0999-193X
DOI: 10.1186/s12711-018-0431-9⟩
Popis: Background Bacterial cold-water disease, which is caused by Flavobacterium psychrophilum, is one of the major diseases that affect rainbow trout (Oncorhynchus mykiss) and a primary concern for trout farming. Better knowledge of the genetic basis of resistance to F. psychrophilum would help to implement this trait in selection schemes and to investigate the immune mechanisms associated with resistance. Various studies have revealed that skin and mucus may contribute to response to infection. However, previous quantitative trait loci (QTL) studies were conducted by using injection as the route of infection. Immersion challenge, which is assumed to mimic natural infection by F. psychrophilum more closely, may reveal different defence mechanisms. Results Two isogenic lines of rainbow trout with contrasting susceptibilities to F. psychrophilum were crossed to produce doubled haploid F2 progeny. Fish were infected with F. psychrophilum either by intramuscular injection (115 individuals) or by immersion (195 individuals), and genotyped for 9654 markers using RAD-sequencing. Fifteen QTL associated with resistance traits were detected and only three QTL were common between the injection and immersion. Using a model that accounted for epistatic interactions between QTL, two main types of interactions were revealed. A “compensation-like” effect was detected between several pairs of QTL for the two modes of infection. An “enhancing-like” interaction effect was detected between four pairs of QTL. Integration of the QTL results with results of a previous transcriptomic analysis of response to F. psychrophilum infection resulted in a list of potential candidate immune genes that belong to four relevant functional categories (bacterial sensors, effectors of antibacterial immunity, inflammatory factors and interferon-stimulated genes). Conclusions These results provide new insights into the genetic determinism of rainbow trout resistance to F. psychrophilum and confirm that some QTL with large effects are involved in this trait. For the first time, the role of epistatic interactions between resistance-associated QTL was evidenced. We found that the infection protocol used had an effect on the modulation of defence mechanisms and also identified relevant immune functional candidate genes. Electronic supplementary material The online version of this article (10.1186/s12711-018-0431-9) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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