The Profile of Toll-like Receptor 2 (TLR2), TLR4 and Their Cytosolic Downstream Signaling Pathway in Common Variable Immunodeficiency (CVID) Patients
| Autor: | Sharifi, L., Aghamohammadi, A., Rezaei, N., Yazdani, R., Mahmoudi, M., Amiri, M. M., Masoumi, F., Saied Bokaie, Tavasolian, P., Sanaei, R., Moshiri, M., Tavakolinia, N., Alinia, T., Azizi, G., Mirshafiey, A. |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Adolescent Myeloid differentiation primary response 88 (MyD88) Common variable immunodeficiency (CVID) B-Lymphocyte Subsets Toll interacting protein (Tollip) lcsh:Medicine Gene Expression Autoimmunity Pneumococcal Vaccines Young Adult Humans B-Lymphocytes lcsh:R Intracellular Signaling Peptides and Proteins Flow Cytometry Toll-Like Receptor 2 Toll-Like Receptor 4 Common Variable Immunodeficiency Myeloid Differentiation Factor 88 Leukocytes Mononuclear Female Toll-like receptor 2 (TLR2) Toll-like receptor 4 (TLR4) Signal Transduction |
| Zdroj: | Scopus-Elsevier Iranian Journal of Allergy, Asthma and Immunology, Vol 17, Iss 2 (2018) |
| ISSN: | 1735-1502 |
| Popis: | Common variable immunodeficiency (CVID) is the most common clinical primary antibody deficiency, characterized by increased susceptibility to recurrent bacterial infections. Since Toll-like receptors (TLRs) play an important role in the maturation and differentiation of B-cells, TLRs’ defect can be involved in the pathogenesis of CVID. Therefore, we evaluated the expression of TLR2 and TLR4 and their signaling pathway; also their association with autoimmunity, B-cell subtypes and response to pneumovax-23 were assessed in CVID patients. Sixteen CVID patients were enrolled in the study. Flow cytometry was used for assessing the protein expression of TLR2 and TLR4, and real-time PCR was used for gene expression of myeloid differentiation primary response 88 (MyD88) and toll interacting protein (Tollip). We found a higher protein expression of TLR2 in CVID patients which was associated with lower number of end stage B-cells and hyporesponse to pneumovax-23 vaccination. We showed a lower mRNA expression of MyD88 and an almost equal Tollip mRNA expression in CVID patients compared with controls. There was a profound association between MyD88 gene expression and autoimmunity in CVID patients. According to the presence of the lower number of end stage B-cells and poor vaccine response in CVID patients and their correlation with the higher expression of TLR2, we hypothesized that there is a functional defect in this receptor and/or its downstream in the peripheral blood mononuclear cells (PBMCs) of CVID patients. |
| Databáze: | OpenAIRE |
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