Dot-enzyme-linked immunosorbent assay (Dot-ELISA) of anti-Entamoeba histolytica antibodies in human serum and colostrum

Autor:	López Revilla R, Navarro-García F, Valadez-Sánchez M, Yolanda López-Vidal, Calva Mercado J
Rok vydání:	1991
Předmět:	Adult Male Entamoebiasis Colostrum Entamoeba histolytica Infant Newborn Antibodies Protozoan Enzyme-Linked Immunosorbent Assay Puerperal Disorders Pregnancy Immunoglobulin G Diarrhea Infantile Immunoglobulin A Secretory Liver Abscess Amebic Animals Humans Female Rabbits
Zdroj:	Scopus-Elsevier Europe PubMed Central
ISSN:	0066-6769
Popis:	Seroepidemiologic and clinical studies of amoebiasis require improved methods for the detection and titration of anti-Entamoeba histolytica (Eh) trophozoite antibodies. We have developed a Dot-enzyme-linked immunosorbent assay (Dot-ELISA) to titrate anti-Eh antibodies in human serum and colostrum. The anti-Eh serum IgG antibody titers from healthy individuals and from patients with amoebic liver abscess obtained, with our Dot-ELISA were similar to those obtained with a standard ELISA, but the background values were lower. Significant anti-Eh sIgA antibody titers were found in seven out of 42 colostrum samples of mothers of newborn children with diarrhea. Our Dot-ELISA is rapid, inexpensive, and might be used for the diagnosis of individuals and in seroepidemiologic studies of amoebiasis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::5c73def7c9ecd45c25ced43f6abc30bd https://pubmed.ncbi.nlm.nih.gov/1844108 Zobrazit plný text záznamu