Reverse transcription-polymerase chain reaction analysis of genes involved in gonadal differentiation in pigs
| Autor: | Parma, P., Pailhoux, E., Cotinot, C. |
|---|---|
| Přispěvatelé: | Unité de biologie cellulaire et moléculaire, Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Anti-Mullerian Hormone
Male Swine [SDV]Life Sciences [q-bio] Gene Expression Gestational Age Aromatase Receptors Gonadotropin Testis Animals RNA Messenger Gonads ComputingMilieux_MISCELLANEOUS Glycoproteins Sertoli Cells Reverse Transcriptase Polymerase Chain Reaction Ovary High Mobility Group Proteins Nuclear Proteins Cell Differentiation SOX9 Transcription Factor Sex Determination Processes Growth Inhibitors Sex-Determining Region Y Protein DNA-Binding Proteins [SDV] Life Sciences [q-bio] Testicular Hormones Female Transcription Factors |
| Zdroj: | Biology of Reproduction Biology of Reproduction, Society for the Study of Reproduction, 1999, 61, pp.741-748 |
| ISSN: | 0006-3363 1529-7268 |
| Popis: | In mammals, testis development is initiated in the embryo as a response to the expression of the sex-determining gene, SRY. The time course of SRY expression during gonadal differentiation in the male has been described in detail only in mice and sheep. In this study, we used reverse transcription-polymerase chain reaction analysis to define the SRY transcription profile in pig genital ridges. SRY transcripts were first detectable from 23 days postcoitum (dpc), then declined sharply after 35 dpc. None were detected at 60 dpc. In addition, we analyzed temporal expression of other genes known to be involved in mammalian sex determination: WT-1, SF-1, SOX9, and AMH. A key stage seems to be 28 dpc, in which SOX9 expression switches between the male and female, and AMH expression begins to attest to Sertoli cell differentiation and to correspond to seminiferous cord formation in the male. Expression of gonadotropin receptors and aromatase was also investigated in porcine gonads, and we showed that their transcripts were detected very early on, especially in the male: 25 dpc for the LH receptor (rLH) and aromatase, and 28 dpc for the FSH receptor (rFSH). In the female, aromatase transcripts were not detected until 70 dpc, and rFSH expression occurred later: at 45 dpc at the onset of meiosis. Moreover, no difference was observed between the sexes for the onset of rLH transcription at 25 dpc. Such a thorough study has never been performed on pigs; developmental analysis will be useful for investigating sex-reversed gonads and determining ontogeny in intersexuality, a common pathology in pigs. |
| Databáze: | OpenAIRE |
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