Ubiquitin-activating enzyme is necessary for 17β-estradiol-induced breast cancer cell proliferation and migration
| Autor: | PESIRI, VALERIA, TOTTA, PIERANGELA, MARINO, Maria, ACCONCIA, FILIPPO |
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| Přispěvatelé: | Pesiri, V, Totta, P, Marino, Maria, Acconcia, Filippo, Totta, Pierangela, Pesiri, Valeria |
| Rok vydání: | 2014 |
| Předmět: |
Blotting
Western Breast Neoplasms Ubiquitin-Activating Enzymes Benzoates Benzoate Pyr-41 breast cancer MCF-7 Cell Cell Movement Furan Humans Furans Cell Proliferation Analysis of Variance Wound Healing Microscopy Confocal Estradiol 17β-estradiol Pyrazole Ubiquitin-Activating Enzyme MCF-7 Cells Pyrazoles Female Breast Neoplasm estrogen receptor Human Signal Transduction |
| Zdroj: | IUBMB life. 66(8) |
| ISSN: | 1521-6551 |
| Popis: | The sex steroid hormone 17β-estradiol (E2) regulates breast cancer (BC) cell proliferation and migration through the activation of a plethora of signal transduction cascades (e.g., PI3K/AKT activation) starting after E2 binding to the estrogen receptor alpha (ERα). The activity of the ubiquitin (Ub)-system modulates many physiological processes (e.g., cell proliferation and migration), and recently, a specific inhibitor (Pyr-41) of the Ub-activating enzyme (E1), which works as the activator of the Ub-based signaling, has been identified to prevent the functions of the Ub-system. Here, by using Pyr-41, we studied the involvement of the Ub-system in E2-induced signaling to proliferation and migration of BC cells. Our data indicate that E1 activity is involved in the E2:ERα signaling important for cell proliferation and migration through the modulation of the E2-evoked activation of the PI3K/AKT and the p38/MAPK pathways. These discoveries indicate a new molecular circuitry that can be further explored to define new opportunities for BC treatment. |
| Databáze: | OpenAIRE |
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