Adherent invasive Escherichia coli (AIEC) strain LF82, but not Candida albicans, plays a profibrogenic role in the intestine
| Autor: | Dina Chokr, Marjorie Cornu, Christel Neut, Clovis Bortolus, Rogatien Charlet, Pierre Desreumaux, Silvia Speca, Boualem Sendid |
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| Přispěvatelé: | Centre de recherche sur l'Inflammation (CRI (UMR_S_1149 / ERL_8252 / U1149)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Lille Inflammation Research International Center - U 995 (LIRIC), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Lille, LillOA, CHU Lille, CNRS, Inserm, Université de Lille, Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286, Centre de recherche sur l'Inflammation [CRI (UMR_S_1149 / ERL_8252 / U1149)], Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576, Lille Inflammation Research International Center (LIRIC) - U995 |
| Rok vydání: | 2020 |
| Předmět: |
[SDV]Life Sciences [q-bio]
Research C. albicans AIEC strain LF82 TGF-beta-stimulated intestinal epithelial cells Inflammatory bowel disease Intestinal fibrosis digestive system digestive system diseases [SDV] Life Sciences [q-bio] lcsh:Diseases of the digestive system. Gastroenterology lcsh:RC799-869 TGF-β-stimulated intestinal epithelial cells |
| Zdroj: | Gut Pathogens Gut Pathogens, 2021, Gut pathogens, 13, pp.5. ⟨10.1186/s13099-021-00401-z⟩ Gut Pathogens, Vol 13, Iss 1, Pp 1-10 (2021) |
| ISSN: | 1757-4749 |
| Popis: | International audience; BACKGROUND: Intestinal fibrosis is a frequent complication of Crohn's disease. However, the factors that cause chronicity and promote fibrogenesis are not yet understood.OBJECTIVE: In the present study, we evaluated the profibrotic effects of adherent-invasive Escherichia coli (AIEC) LF82 strain and Candida albicans in the gut.METHODS: Colonic fibrosis was induced in C57BL/6 mice by administration of three cycles of 2.5% (w/v) dextran sulfate sodium (DSS) for 5 weeks. LF82 and C. albicans were administered orally once at the start of each week or each cycle, respectively. Expression of markers of myofibroblast activation was determined in TGF-β1-stimulated human intestinal epithelial cells (IECs).RESULTS: LF82 administration exacerbated fibrosis in DSS-treated mice, revealed by increased colonic collagen deposition and expression of the profibrotic genes Col1a1, Col3a1, Fn1 and Vim. This was accompanied by enhanced gene expression of proinflammatory cytokines and chemokines, as well as more recruited inflammatory cells into the intestine. LF82 also potentiated TGF-β1-stimulated epithelial-mesenchymal transition and myofibroblast activation in IECs, by further inducing gene expression of the main mesenchymal cell markers FN1 and VIM and downregulating the IEC marker OCLN. Proinflammatory cytokines were overexpressed with LF82 in TGF-β1-stimulated IECs. Conversely, C. albicans did not affect intestinal fibrosis progression in DSS-treated mice or myofibroblast activation in TGF-β1-stimulated IECs.CONCLUSIONS: These results demonstrate that AIEC strain LF82, but not C. albicans, may play a major profibrogenic role in the gut. |
| Databáze: | OpenAIRE |
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