Short-time QiBaoMeiRan Formula Treatment Exerts Estrogenic Activities without Side Effects on Reproductive Tissues in Immature Mice
| Autor: | Xu, Ying, Ma, Xiao-ping, An, Jin-na, Zhang, Zi-jia, Ding, Jie, Qu, Ya-kun, Liu, Zhen-li, Lin, Na |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Mucous Membrane
urogenital system Reproduction Uterus Estrogens Luteinizing Hormone Article Mice Ki-67 Antigen Retraction Note Estrogen Receptor Modulators Gene Expression Regulation Receptors Estrogen Cell Line Tumor Proliferating Cell Nuclear Antigen Vagina Animals Humans Female Follicle Stimulating Hormone hormones hormone substitutes and hormone antagonists Biomarkers Cell Proliferation Drugs Chinese Herbal |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep17436 |
| Popis: | The Chinese herbal preparation QiBaoMeiRan formula (QBMR) displayed estrogenic effects in ovariectomized rats after long-term administration in a previous study. The uterus and vagina are negatively influenced by estrogens in hormone therapy. While QBMR is known to be a phytoestrogen, its estrogenic effects and safety on reproductive tissues after short-term administration and its mechanism via estrogen receptor (ER) pathway haven’t been studied. Here, we characterized its estrogenic effects using immature mice together with in vitro studies for further molecular characterization. Immature mice were treated with QBMR at doses of 1.125, 2.25, and 4.5 g/kg for 7 days. 1.125 and 2.25 g/kg QBMR promoted the growth and development of uterus and vagina, and upregulated ERα and ERβ expression in reproductive tissues. QBMR had a stimulatory effect on proliferating cell nuclear antigen in vagina but not in uterus, and was without any influence on ki-67 antigen in uterus and vagina. QBMR significantly induced luciferase expression from the ERα/β-estrogen response element (ERE) luciferase reporter and upregulated ERα and ERβ expressions in MCF-7 cells, which were significantly inhibited by estrogen antagonist ICI182,780. This study demonstrated QBMR exerts estrogenic effects on reproductive tissues without side effects and through ER-ERE-dependent pathway. |
| Databáze: | OpenAIRE |
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