A study on the role of nitric oxide and iron in 3-morpholinosydnonimine-induced increases in dopamine release in the striatum of freely moving rats
| Autor: | Serra, P. A., Rocchitta, G., Esposito, G., Delogu, M. R., Rossana MIGHELI, Miele, E., Desole, M. S., Miele, M. |
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| Předmět: |
Male
Dose-Response Relationship Drug Metalloporphyrins Dopamine Iron Movement Homovanillic Acid Ascorbic Acid Free Radical Scavengers Deferoxamine Nitric Oxide Corpus Striatum Acetylcysteine Rats Uric Acid Dialysis Solutions Molsidomine Papers 3 4-Dihydroxyphenylacetic Acid Animals Nitric Oxide Donors Rats Wistar |
| Zdroj: | Scopus-Elsevier |
| Popis: | 1. We showed previously that interaction between NO and iron (II), both released following the decomposition of sodium nitroprusside (SNP), accounted for the late SNP-induced dopamine (DA) increase in dialysates from the striatum of freely moving rats; in addition, we showed that co-infusion of iron (II) with the NO-donor S-nitroso-N-acetylpenicillamine mimicked SNP effects on striatal DA release. 2. In the present study, intrastriatal co-infusion of iron (II) (given as FeSO(4), 1 mM for 40 min) with the NO-donor and potential peroxynitrite generator 3-morpholinosydnonimine (SIN-1) (0.2, 0.5, 1.0 or 5.0 mM for 180 min), potentiated the SIN-1-induced increase in DA concentration in dialysates from the striatum of freely moving rats. Neither alone nor associated with iron (II) did SIN-1 induce changes in dialysate ascorbic acid or uric acid concentrations. 3. Neither co-infusion of a superoxide dismutase mimetic nor uric acid affected SIN-1-induced increases in dialysate DA concentration. 4. Infusion of the iron chelator deferoxamine (0.2 mM for 180 min) decreased dialysate DA and attenuated SIN-1-induced increases in dialysate DA concentrations. 5. These results suggest that iron plays a key role in SIN-1-induced release of striatal DA and do not support any role for either peroxynitrite or superoxide anion in SIN-1-induced release of striatal DA. |
| Databáze: | OpenAIRE |
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