Bacterial transformation: ComFA is a DNA-dependent ATPase that forms complexes with ComFC and DprA

Autor: Diallo, Amy, Foster, Hannah, Gromek, Katarzyna, Perry, Thomas, Dujeancourt, Annick, Krasteva, Petya, Gubellini, Francesca, Falbel, Tanya, Burton, Briana, Fronzes, Rémi
Přispěvatelé: Biologie Structurale de la Sécrétion Bactérienne, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC), University of Wisconsin-Madison, This work was funded by the Centre National de la Recherche Scientifique and the European Research Council (MolStructTransfo, 281149) to RF and by a grant from the Rita Allen Foundation to BMB., European Project: 281149,EC:FP7:ERC,ERC-2011-StG_20101109,MOLSTRUCTTRANSFO(2012), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris]
Jazyk: angličtina
Rok vydání: 2017
Předmět:
[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]
DNA
Single-Stranded
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
MESH: Homologous Recombination
Bacterial Proteins
MESH: Adenosine Triphosphatases
[CHIM.CRIS]Chemical Sciences/Cristallography
MESH: Protein Binding
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Homologous Recombination
MESH: Bacterial Proteins
Adenosine Triphosphatases
Recombination
Genetic
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Structural Biology [q-bio.BM]
MESH: DNA
Membrane Proteins
DNA
DNA-Binding Proteins
Rec A Recombinases
Streptococcus pneumoniae
MESH: DNA
Single-Stranded
MESH: Rec A Recombinases
MESH: Recombination
Genetic
Transformation
Bacterial
MESH: Membrane Proteins
[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM]
MESH: Transformation
Bacterial
MESH: Streptococcus pneumoniae
MESH: DNA-Binding Proteins
Protein Binding
Zdroj: Molecular Microbiology
Molecular Microbiology, 2017, 105 (5), pp.741-754. ⟨10.1111/mmi.13732⟩
Molecular Microbiology, Wiley, 2017, 105 (5), pp.741-754. ⟨10.1111/mmi.13732⟩
ISSN: 0950-382X
1365-2958
Popis: International audience; Pneumococcal natural transformation contributes to genomic plasticity, antibiotic resistance development and vaccine escape. Streptococcus pneumoniae, like many other naturally transformable species, has evolved sophisticated protein machinery for the binding and uptake of DNA. Two proteins encoded by the comF operon, ComFA and ComFC, are involved in transformation but their exact molecular roles remain unknown. In this study, we provide experimental evidence that ComFA binds to single stranded DNA (ssDNA) and has ssDNA-dependent ATPase activity. We show that both ComFA and ComFC are essential for the transformation process in pneumococci. Moreover, we show that these proteins interact with each other and with other proteins involved in homologous recombination, such as DprA, thus placing the ComFA-ComFC duo at the interface between DNA uptake and DNA recombination during transformation.
Databáze: OpenAIRE
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