Bacterial transformation: ComFA is a DNA-dependent ATPase that forms complexes with ComFC and DprA
Autor: | Diallo, Amy, Foster, Hannah, Gromek, Katarzyna, Perry, Thomas, Dujeancourt, Annick, Krasteva, Petya, Gubellini, Francesca, Falbel, Tanya, Burton, Briana, Fronzes, Rémi |
---|---|
Přispěvatelé: | Biologie Structurale de la Sécrétion Bactérienne, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC), University of Wisconsin-Madison, This work was funded by the Centre National de la Recherche Scientifique and the European Research Council (MolStructTransfo, 281149) to RF and by a grant from the Rita Allen Foundation to BMB., European Project: 281149,EC:FP7:ERC,ERC-2011-StG_20101109,MOLSTRUCTTRANSFO(2012), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] |
Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph]
DNA Single-Stranded [SDV.BC]Life Sciences [q-bio]/Cellular Biology MESH: Homologous Recombination Bacterial Proteins MESH: Adenosine Triphosphatases [CHIM.CRIS]Chemical Sciences/Cristallography MESH: Protein Binding [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Homologous Recombination MESH: Bacterial Proteins Adenosine Triphosphatases Recombination Genetic [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] MESH: DNA Membrane Proteins DNA DNA-Binding Proteins Rec A Recombinases Streptococcus pneumoniae MESH: DNA Single-Stranded MESH: Rec A Recombinases MESH: Recombination Genetic Transformation Bacterial MESH: Membrane Proteins [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM] MESH: Transformation Bacterial MESH: Streptococcus pneumoniae MESH: DNA-Binding Proteins Protein Binding |
Zdroj: | Molecular Microbiology Molecular Microbiology, 2017, 105 (5), pp.741-754. ⟨10.1111/mmi.13732⟩ Molecular Microbiology, Wiley, 2017, 105 (5), pp.741-754. ⟨10.1111/mmi.13732⟩ |
ISSN: | 0950-382X 1365-2958 |
Popis: | International audience; Pneumococcal natural transformation contributes to genomic plasticity, antibiotic resistance development and vaccine escape. Streptococcus pneumoniae, like many other naturally transformable species, has evolved sophisticated protein machinery for the binding and uptake of DNA. Two proteins encoded by the comF operon, ComFA and ComFC, are involved in transformation but their exact molecular roles remain unknown. In this study, we provide experimental evidence that ComFA binds to single stranded DNA (ssDNA) and has ssDNA-dependent ATPase activity. We show that both ComFA and ComFC are essential for the transformation process in pneumococci. Moreover, we show that these proteins interact with each other and with other proteins involved in homologous recombination, such as DprA, thus placing the ComFA-ComFC duo at the interface between DNA uptake and DNA recombination during transformation. |
Databáze: | OpenAIRE |
Externí odkaz: |