Genomic modifications in naturally occurring neurovirulent revertants of Sabin 1 polioviruses

Autor:	Otelea, D., Guillot, S., Furione Milena, Combiescu, A. A., Balanant, J., Candrea, A., Crainic, R.
Rok vydání:	1993
Předmět:	Poliovirus Virulence Poliovirus Vaccine Oral Mutation Humans Infant RNA Viral Polymerase Chain Reaction Polymorphism Restriction Fragment Length Poliomyelitis
Zdroj:	Europe PubMed Central Scopus-Elsevier
ISSN:	0301-5149
Popis:	Five type 1 Sabin-like poliovirus strains were isolated from paralytic cases of poliomyelitis. Their Sabin origin was confirmed by antigenic analysis with monoclonal antibodies and restriction fragment length polymorphism (RFLP) assay. Several nucleotide positions known to play a role in the reversion towards neurovirulence were examined: viz 480, 2741, 6203, 7441. Analysis was performed by enzymatic restriction and partial sequencing of PCR-amplified genomic segments. All the strains bore the reversion GA at residue 480; in four of the five viruses, only this reversion was found. The fifth strain presented four additional reversions and several new mutations. These results indicate that, during multiplication in the human gut, Sabin 1 poliovirus carrying one or several mutations related to reversion towards neurovirulence can be selected.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::026ccfa035d5c8d03732055a0e4832e1 https://pubmed.ncbi.nlm.nih.gov/8099049 Zobrazit plný text záznamu