| Autor: |
V V, Bezuglov, E V, Fomina-Ageeva, N M, Gretskaia, E V, Kriukova, E V, Diatlovitskaia, L D, Bergel'son |
| Rok vydání: |
1991 |
| Předmět: |
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| Zdroj: |
Biokhimiia (Moscow, Russia). 56(2) |
| ISSN: |
0320-9725 |
| Popis: |
Using reverse phase high performance chromatography with UV-detection, the arachidonic acid cascade in human peripheral blood lymphocytes (PBL) was studied. It was found that PBL oxidized arachidonic acid via the lipoxygenase pathway, 12-hydroxyeicosatetraenoic acid (12-HETE) being the major metabolite of endogenous arachidonic acid. Exogenous arachidonic acid added to human PBL suspensions increased 12-HETE synthesis 5-7 times. In another experimental series the effects of gangliosides (GD3, GM1 and GM3) on lipoxygenase-catalyzed oxidation of arachidonic acid in human lymphocytes were investigated. All the gangliosides tested stimulated PBL to secrete 12-HETE both from endogenous and exogenous arachidonic acid. In most cases the stimulating effect of GD3 was much more apparent that those of GM1 and GM3. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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