| Autor: |
Valeriy, Lukyanenko, Vadim, Salnikov |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 991 |
| ISSN: |
1940-6029 |
| Popis: |
Delivery of nano-objects to certain intracellular sub-domains is crucial for nanomedicine. Therefore delivery of nano-object to desirable cellular compartment has to be confirmed. The most valuable confirmation of the delivery comes from direct visualization of the nano-object. This visualization usually requires use of microscope and corresponding probe which has to be conjugated with the nano-object. There are two most popular methods of the visualization: confocal and electron microscopy. The former has significant limitations due to diffraction limited resolution of confocal systems and three-dimensional convolution of fluorescence. The latter should be significantly modified for needs of the visualization. Here we describe the method for precise localization of nano-object within the cell using electron microscopy and 1-2 nm gold particles as a nanomarker. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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