Attenuation of Borrelia anserina by serial passage in liquid medium

Autor: J F, Levine, M J, Dykstra, W L, Nicholson, R L, Walker, G, Massey, H J, Barnes
Rok vydání: 1990
Předmět:
Zdroj: Research in veterinary science. 48(1)
ISSN: 0034-5288
Popis: Borrelia anserina (Sakharoff) was successfully grown in a liquid medium (Barbour-Stoenner-Kelly) for 39 passages. By the 12th serial passage in medium, infectivity of B anserina for chicks was lost. Electron microscopy did not reveal structural differences between non-infective and infective cultured organisms. Changes in the protein profiles were found by electrophoresis as the organisms were passed in culture.
Databáze: OpenAIRE