Chemical forms of selenium present in rat and ram spermatozoa

Autor:	N S, Alabi, M A, Beilstein, P D, Whanger
Rok vydání:	2000
Předmět:	Male Glutathione Peroxidase Sheep Hydrolysis Selenium Radioisotopes Proteins Chromatography Ion Exchange Spermatozoa Rats Selenocysteine Rats Sprague-Dawley Selenium Sodium Selenite Chromatography Gel Animals Amino Acids Selenoproteins
Zdroj:	Biological trace element research. 76(2)
ISSN:	0163-4984
Popis:	In vivo and in vitro studies were conducted to investigate the chemical forms by ion-exchange chromatography of selenium (Se) present in rat and ovine spermatozoa. After injection with 75Se-selenite, the form of 75Se in rat sperm was selenocysteine, but selenocysteine and selenomethionine (SeMet) were present in ovine sperm. Presumably, synthesis of SeMet by rumen microbes are responsible for its presence in ovine sperm. In vitro incubation of ram sperm with selenocysteine or SeMet produced no changes, but incubation with selenite produced a compound that eluted one fraction before SeMet from the ion-exchange column. After treatment of this fraction with mercaptoethanol, it eluted in a later fraction upon rechromatography, suggesting it to be selenodicysteine. This compound is apparently formed because of high levels of cysteine in semen. Cysteine, reduced glutathione, and oxidized glutathione were also found in semen. The significance of the results is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::faecc62713464d6eb832b34cc36baaa4 https://pubmed.ncbi.nlm.nih.gov/11049230 Zobrazit plný text záznamu Full text from SpringerLink