Immobilized endoproteinase Glu-C to magnetic bead cellulose as a tool in proteomic analysis

Autor:	Petr, Prikryl, Marie, Ticha, Zdenka, Kucerova
Rok vydání:	2013
Předmět:	Proteomics Magnetics Staphylococcus aureus Bacterial Proteins Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Enzyme Stability Serine Endopeptidases Caseins Serum Albumin Bovine Hydrogen-Ion Concentration Cellulose Enzymes Immobilized
Zdroj:	Journal of separation science. 36(12)
ISSN:	1615-9314
Popis:	Magnetic bead cellulose activated with divinyl sulfone was used for the immobilization of Staphylococcus aureus endoproteinase Glu-C (EC 3.4.21.19). The immobilized proteinase was characterized by increased thermostability, by decreased self-cleavage activity, and a possibility of repeated use. The prepared immobilized enzyme was applied for the proteolytic cleavage of α-casein and BSA under different conditions (different composition of buffers, different pH, and different time of digestion). The possibilities of the direct use of enzyme reaction products for MALDI TOF MS analysis were shown.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::fac250f04e9add563c409bbf24d044be https://pubmed.ncbi.nlm.nih.gov/23576383 Zobrazit plný text záznamu Plný text