| Autor: |
Chengshan, Xu, Ling, Zhang, Hengheng, Li, Zhihua, Liu, Lianning, Duan, Chengrong, Lu |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
American journal of cancer research. 5(3) |
| ISSN: |
2156-6976 |
| Popis: |
MicroRNAs play key roles in cell growth, differentiation, and apoptosis. In this study, we described the regulation and function of miR-1469 in apoptosis of lung cancer cells (A549 and NCI-H1650). Expression analysis verified that miR-1469 expression significantly increased in apoptotic cells. Overexpression of miR-1469 in lung cancer cells increased cell apoptosis induced by etoposide. Additionally, we identified that Stat5a is a downstream target of miR-1469, which can bind directly to the 3’-untranslated region of the Stat5a, subsequently reducing both the mRNA and protein levels of Stat5a. Finally, co-expression of miR-1469 and Stat5a in A549 and NCI-H1650 cells partially abrogated the effect of miR-1469 on cell apoptosis. Our results show that miR-1469 functions as an apoptosis enhancer to regulate lung cancer apoptosis through targeting Stat5a and may become a critical therapeutic target in lung cancer. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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