Characterization of the recombinant Clostridium pasteurianum ferredoxin and comparison of its properties with those of the native protein

Autor:	J R, Baur, M C, Graves, B A, Feinberg, S W, Ragsdale
Rok vydání:	1990
Předmět:	Clostridium Electron Transport Bacterial Proteins Base Sequence Molecular Sequence Data Escherichia coli Ferredoxins Oxidation-Reduction Recombinant Proteins
Zdroj:	BioFactors (Oxford, England). 2(3)
ISSN:	0951-6433
Popis:	Ferredoxins (Fds) constitute an important class of nonheme iron-sulfur proteins. One of the most studied Fds is the [8Fe-8S] Fd from Clostridium pasteurianum. The gene for this Fd has previously been cloned and sequenced. We report the expression of this Fd in Escherichia coli, and the characterization and comparison of this recombinant protein to the native Fd. We have found that the purified recombinant protein has the same enzymatic, redox, magnetic and electronic properties as the native Fd isolated from C. pasteurianum, which indicates that the two [4Fe-4S] clusters present in the Fd were correctly formed in E. coli.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::f99c7c3484d9c5f9b0b76a420e52a1b6 https://pubmed.ncbi.nlm.nih.gov/2198878 Zobrazit plný text záznamu