| Autor: |
Ke, Wang, Guo-Guang, Peng, Yu-Lian, Tan, Shan-Zhi, He, Cui-Fen, Luo |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Shanghai kou qiang yi xue = Shanghai journal of stomatology. 30(1) |
| ISSN: |
1006-7248 |
| Popis: |
To investigate miR-99a expression and its effect on proliferation of oral squamous cell carcinoma (OSCC).miRNA microarrays associated with OSCC were identified in GEO database. The expression levels of miR-99a were detected in 63 OSCC tissues and adjacent normal tissues and cell lines. The relationship between clinicopathological parameters and miR-99a expression was analyzed by using ANOVA analysis. The ability of cell growth and clone formation were examined in SCC9 and SCC25 cells transfected with miR-99a mimics. The target genes of miR-99a were predicted by Targetscan software. There resulting data were analyzed using SPSS 19.0 software package.The differently expressed miRNAs were analyzed based on GSE103931 microarray. miR-99a was significantly downregulated in OSCC tissues and cell lines. miR-99a expression was significantly associated with T stage, pathological grading and patients' prognosis. miR-99a overexpression inhibited OSCC cell proliferation and clone formation, while miR-99a inhibition contributed to decreased proliferation and clone formation ability. In addition, miR-99a combined with mTOR gene's 3'UTR was negatively correlated with mTOR expression in OSCC tissues.miR-99a functions as a tumor suppressor in OSCC and inhibits OSCC cell proliferation by targeting mTOR. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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