| Autor: |
Q, Sun, C-W, Jiang, Z-H, Tan, L-Y, Hou, H, Dong, K, Liu, G, Sun, Y-J, Liu, Y-Q, Wang, X-C, Lu, H, Liu |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
European review for medical and pharmacological sciences. 21(10) |
| ISSN: |
2284-0729 |
| Popis: |
The lung adenocarcinoma is a type of lung cancer. This research is to investigate the effects of miR-222 on the proliferation, migration and invasion of the lung adenocarcinoma cells.At the beginning, MiR-222 and the controls were transfected to the lung adenocarcinoma cell line A549 for CCK-8 proliferation, transwell migration and Matrigel invasion, and then observed the effect of miR-222 on the proliferation, migration and invasion of lung adenocarcinoma cells. The miR-222 target was regulated by ETS1 downwards to participate in the regulation of the process by using the luciferase reporter assay, the Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and the Western blotting.According to CCK-8 proliferation assay, the Transwell migration and the Matrigel invasion assay, it discovered that MiR-222 can promote the proliferation, migration and invasion of the lung adenocarcinoma cells. Luciferase reporter assay, RT-qPCR and Western blot assay showed that miR-222 could regulate the expression of ETS1 downwards and ETS1 participated in the regulation of the process CONCLUSIONS: ETS1 promotes proliferation, migration and invasion of lung adenocarcinoma cells by targeting the regulated miR-222 downwards. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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