| Autor: |
Friedhelm R, Schuster, Raymund, Buhmann, Susanne, Reuther, Bernd, Hubner, Christine, Grabrucker, Anja, Liepert, Roland, Reibke, Peter, Lichtner, Ting, Yang, Tanja, Kroell, Hans-Jochem, Kolb, Arndt, Borkhardt, Helga, Schmetzer |
| Rok vydání: |
2009 |
| Předmět: |
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| Zdroj: |
Cancer genomicsproteomics. 5(5) |
| ISSN: |
1109-6535 |
| Popis: |
Recently it was shown that myeloid leukemic cells can be induced to differentiate into leukemia-derived dendritic cells (DCleu), regaining the stimulatory capacity of professional DCs while presenting the leukemic antigen repertoire. But so far, the induced antileukemic T-cell responses have varied in specificity and efficacy, or have even mediated opposite effects. In an attempt to further characterize the DC/DCleu induced T-cell response pattern, immunoscope spectratyping, a novel and powerful tool to detect T-cell receptor (TCR) rearrangements was used in combination with functional flow cytometry and non-radioactive fluorolysis assays. Human leucocyte antigen (HLA) matched donor T-cells were repeatedly stimulated, either with leukemic blasts (French-American-British, FAB M4eo) or the corresponding blast-derived DCs. Functional comparison revealed no significant difference in their T-cell stimulatory capacity, while the DC/DCleu fraction favored T-cells with a higher lytic activity, comprising a higher proportion of T-memory CD45R0+ cells. Stimulation with blasts and DC/DCleu induced a similar TCR restriction pattern, while stimulation with DC/DCleu favored the CD4 T-cell subset and seemed to cause a higher grade of restriction. In conclusion, a combined strategy using spectratyping with functional tests might not only provide useful information about the specificity and efficacy of the induced T-cell response, but also pave the way to gain effective T-cell clones for therapeutic use. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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