Strategy for protein isoform identification from expressed sequence tags and its application to peptide mass fingerprinting

Autor:	F C, Lisacek, M D, Traini, D, Sexton, J L, Harry, M R, Wilkins
Rok vydání:	2001
Předmět:	Expressed Sequence Tags Proteome Lipoxygenase Molecular Sequence Data Proteins Oryza Peptide Mapping Solanum lycopersicum Phosphopyruvate Hydratase Cluster Analysis Protein Isoforms Electrophoresis Gel Two-Dimensional Amino Acid Sequence Soybeans Sequence Alignment Plant Proteins
Zdroj:	Proteomics. 1(2)
ISSN:	1615-9853
Popis:	Expressed Sequence Tags (ESTs) are an invaluable resource for protein identification and characterisation in proteomics. They allow proteins to be identified in the absence of genome sequence data. When EST sequences are used for protein identification, they are usually first processed into contigs to reduce redundancy and generate longer sequences from the overlapping ESTs. However, the process of generating contigs may accidentally group biologically meaningful isoforms together. Here we report means of discovering isoforms in EST sequences and how to use this information in the framework of protein identification and characterisation with peptide mass fingerprinting. We illustrate our strategies with examples from the dbEST database as well as protein isoforms from two-dimensional polyacrylamide gels.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::eeaec23e4fc2e30f8a9880bba052aae5 https://pubmed.ncbi.nlm.nih.gov/11680865 Zobrazit plný text záznamu Plný text