[Construction of RGD10-NGR9 dual-targeting superparamagnetic iron oxide and its magnetic resonance imaging features in nude mice]
| Autor: | Qiong-ya, Wu, Jing-yun, Shi, Jie, Zhang, Lin-qian, Zhang, Yin-min, Zhao, Liang, Tang, Yun, Chen, Xiao-dong, He, Hui, Liu, Bo, Su |
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| Rok vydání: | 2014 |
| Předmět: |
Mice
Inbred BALB C Lung Neoplasms Contrast Media Mice Nude Dextrans Adenocarcinoma Signal-To-Noise Ratio Integrin alphaVbeta3 Aminopeptidases Magnetic Resonance Imaging Ferrosoferric Oxide Mice Cell Line Tumor Human Umbilical Vein Endothelial Cells Animals Humans Particle Size Magnetite Nanoparticles Oligopeptides Cells Cultured Neoplasm Transplantation |
| Zdroj: | Zhonghua zhong liu za zhi [Chinese journal of oncology]. 35(11) |
| ISSN: | 0253-3766 |
| Popis: | To construct angiogenesis-specific RGD10-NGR9 dual-targeting superparamagnetic iron oxide nanoparticles, and to evaluate its magnetic resonamce imaging (MRI) features in nude mice and potential diagnostic value in tumor MRI.Dual-targeting peptides RGD10-NGR9 were designed and synthesized. Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles were synthesized by chemical co-precipitation method and the surface was modified to be hydrophilic by coating with dextran. The dual-targeting peptides RGD10-NGR9 were conjugated to USPIO. Cell binding affinity and up-taking ability of the dual-targeting USPIO nanoparticles to integrin ανβ3-APN positive cells were subsequently tested by Prussian blue staining and phenanthroline colorimetry in vitro. The RGD10-NGR9 conjugated with USPIO was injected intravenously into xenograft mice, which were scanned by MRI at predetermined time points. The MRI and contrast-to-noise ratio (CNR) values were calculated to evaluate the ability of dual-targeting USPIO as a potential contrast agent in nude mice.P-CLN-Dextran-USPIO nanoparticles with stable physical properties were successfully constructed. The average diameter of Fe3O4 nanoparticles was 8-10 nm, that of Dextran-USPIO was about 20 nm and P-CLN-Dextran-USPIO had an average diameter about 30 nm. The in vitro studies showed a better specificity of dual-targeting USPIO nanoparticles on proliferating human umbilical vein endothelia cells (HUVEC). In vivo, RGD10-NGR9-USPIO showed a significantly reduced contrast in signal intensity and 2.83-times increased the CNR in the tumor MRI in xenograft mice.This novel synthesized RGD10-NGR9 dual-targeting USPIO is with better specific affinity in vitro and in vivo, and might be used as a molecular contrast agent for tumor angiogenesis MRI. |
| Databáze: | OpenAIRE |
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