| Autor: |
S G, Graber, R A, Figler, V K, Kalman-Maltese, J D, Robishaw, J C, Garrison |
| Rok vydání: |
1992 |
| Předmět: |
|
| Zdroj: |
The Journal of biological chemistry. 267(19) |
| ISSN: |
0021-9258 |
| Popis: |
The 36-kDa beta 1, 35-kDa beta 2, and 6.5-kDa gamma 2 subunits of the heterotrimeric guanine nucleotide-binding proteins have been overexpressed in Sf9 cells using a baculovirus expression system. The gamma 2 subunit expressed in Sf9 cells incorporated label derived from [3H]mevalonate and is therefore likely to be isoprenylated, as is its mammalian counterpart. Extracts of Sf9 cells doubly infected with viruses encoding a beta subunit and viruses encoding a gamma subunit are active in promoting the pertussis toxin-catalyzed ADP-ribosylation of a G protein alpha subunit. However, extracts from Sf9 cells singly infected with viruses encoding either a beta or gamma subunit are not active in this assay. Results demonstrate utility of the insect/baculovirus system for expressing G protein beta gamma subunits of defined composition. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
