| Autor: |
Antonio J, Ruiz-Sanchez, Claudio, Parolo, Benjamin S, Miller, Eleanor R, Gray, Kristina, Schlegel, Rachel A, McKendry |
| Rok vydání: |
2020 |
| Zdroj: |
Journal of materials chemistry. B. 5(35) |
| ISSN: |
2050-7518 |
| Popis: |
We report the development of a tuneable plasmonic nanochain immunoassay with increased sensitivity over traditional monodisperse nanoparticle lateral flow tests. Our approach takes advantage of the unique self-assembling properties of polyamidoamine dendrimers with gold nanoparticles in aqueous media to create one-dimensional nanochains, with a distinct red to blue colour change, attributable to a longitudinal plasmon resonance, which can be readily detected by eye and a digital camera. We optimise and characterise nanochain formation and stability using UV-visible spectroscopy, transmission electron microscopy and dynamic light scattering. As a proof-of-principle we focus on the application of nanochains for point-of-care diagnostics for p24, an important biomarker of early HIV infections and successfully detect p24 with a limit of detection of 5 ng ml |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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