| Autor: |
Alexandra, Wagner, A Rita, Silva-Santos, Sara Sousa, Rosa, Sophie, Gierak, Ana M, Azevedo, Duarte Miguel F, Prazeres |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2197 |
| ISSN: |
1940-6029 |
| Popis: |
A method for the intermediate recovery of plasmid DNA (pDNA) from alkaline lysates is described that comprises differential isopropanol precipitation steps. In a first low-cut precipitation, a smaller amount of isopropanol (20% v/v) is used so that only high molecular weight RNA precipitates. After solid liquid separation, a high-cut precipitation is performed by bringing isopropanol concentration to 70% v/v to precipitate pDNA. Tests made with lysates show that the differential precipitation increases purity threefold compared to the conventional one-step precipitation at 70% v/v without affecting pDNA recovery (80%). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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