| Autor: |
Pei-Wen, Pan, Shu-Hong, Zhao, Mei, Yu, Bang, Liu, Tong-An, Xiong, Kui, Li |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
Yi chuan xue bao = Acta genetica Sinica. 29(10) |
| ISSN: |
0379-4172 |
| Popis: |
A novel expressed sequence tag (ESThp9-1, GenBank accession number: B1596262) was isolated from pig skeletal muscular tissue by using the mRNA differential display technique. BLAST analysis revealed that the 196 bp long EST (ESThp9-1) was not homologous to any of the known porcine genes in the database but similar to rat U3A small nuclear RNA (87% identity over 93 nucleotides) and mouse U3B.4 small nuclear RNA (85% identity over 96 nucleotides). Semi-quantitative reverse transcription polymerase chain reaction indicated that EST9hp-1 was expressed in most of tissue of the pig. ESThp9-1 was physically mapped on sus scrofa chromosome 12q1.1-q1.5 and linked with microsatellite S0090 by using somatic cell hybrid panel and radiation hybrid panel analysis. According to the homologous information and result of physical mapping, ESThp9-1 was presumed to be one member of the porcine U3 gene family. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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