Chronic imaging and manipulation of cells and vessels through a polished and reinforced thinned-skull

Autor:	Drew, Patrick J., Shih, Andy Y., Driscoll, Jonathan D., Knutsen, Per Magne, Blinder, Pablo, Davalos, Dimitrios, Akassoglou, Katerina, Tsai, Philbert S., Kleinfeld, David
Jazyk:	angličtina
Rok vydání:	2010
Předmět:	Cerebral Cortex Mammals Microscopy Microscopy Confocal Skull Bone Cements Article Imaging Brain Ischemia Mice Cerebrovascular Circulation Animals Wakefulness Cerebrum Blood Flow Velocity Neuroscience
Zdroj:	Nature methods
ISSN:	1548-7105 1548-7091
Popis:	We present a method to form an optical window in the mouse skull that spans millimeters and is stable for months without inflammation of the brain. This enabled us to repeatedly image blood flow in cortical capillaries of awake animals and determine long-range correlations in speed. We further demonstrate repeated cortical imaging of dendritic spines, microglia, and angioarchitecture, as well as illumination to drive motor output via optogenetics and induce microstrokes via photosensitizers.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::e2fe9e4c5fb00160045a2f9e33244504 http://europepmc.org/articles/PMC3204312 Zobrazit plný text záznamu Plný text ve formátu PDF