Determination of ferrocene-A in the blood serum of rabbits using reversed-phase microcolumn HPLC

Autor:	M Sh, Pochkhidze, L P, Asatiani, M D, Rukhadze, Z J, Chitiashvili, M A, Tsartsidze
Rok vydání:	1999
Předmět:	Anti-Infective Agents Metallocenes Animals Reproducibility of Results Antineoplastic Agents Spectrophotometry Ultraviolet Ferrous Compounds Rabbits Sensitivity and Specificity Chromatography High Pressure Liquid
Zdroj:	Biomedical chromatography : BMC. 13(1)
ISSN:	0269-3879
Popis:	Ferrocene-A (ferrocenyl-1-phenyl-1-dioxy-1,4-butin-2) has shown significant antitumour and antibacterial activity. To facilitate studies in this field a simple and rapid liquid chromatographic procedure was developed, allowing the determination of ferrocene-A in the blood serum. The procedure was based on a reversed-phase high-performance liquid chromatography (HPLC) method, with UV detection at 210 nm. The mobile phase ethanol-0.01 M KH2PO4 60:40 (v/v) provides the complete separation of ferrocene-A, internal standard and endogenous compounds of serum. The recovery of ferrocene-A from serum using a hexaneisoamyl alcohol mixture was 90%. The proposed method is convenient for pharmacological and pharmacokinetic applications.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::e2ac44617d88971879ab50e052fa9d6d https://pubmed.ncbi.nlm.nih.gov/10191953 Zobrazit plný text záznamu Plný text