A novel vacuolar protein encoded by SSU21 / MCD4 is involved in cell wall integrity in yeast

Autor:	A N, Packeiser, V N, Urakov, Y A, Polyakova, N I, Shimanova, V D, Shcherbukhin, V N, Smirnov, M D, Ter-Avanesyan
Rok vydání:	1999
Předmět:	Fungal Proteins Saccharomyces cerevisiae Proteins Cell Wall Genes Fungal Mutation Vacuoles Membrane Proteins Saccharomyces cerevisiae Urokinase-Type Plasminogen Activator Protein Kinase C
Zdroj:	Yeast (Chichester, England). 15(14)
ISSN:	0749-503X
Popis:	Using a screening procedure for obtaining yeast strains with enhanced ability to secrete heterologous protein, we have isolated a mutant with alteration of the cell wall structure. This mutant displayed strong decrease in cell wall mannoprotein content, which was not accompanied by decreased glycosylation of secreted proteins. The mutation defines a gene, designated SSU21(identical to previously characterized MCD4), which encodes a novel vacuolar protein. SSU21 is probably connected to the cell integrity protein kinase C-mediated pathway, since ssu21 and pkc1Delta double mutant is synthetic lethal. To our knowledge, this is the first example of a yeast vacuolar protein whose alteration results in a cell wall defect.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::e2126d97f4eb2f675d354d162113260c https://pubmed.ncbi.nlm.nih.gov/10514566 Zobrazit plný text záznamu Plný text