| Autor: |
E, Ayroldi, L, Cannarile, F, D'Adamio, C, Riccardi |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
Immunology. 84(4) |
| ISSN: |
0019-2805 |
| Popis: |
Experiments were performed to evaluate the role of antigen-presenting cells (APC) and the effect of interleukin-4 (IL-4) and glucocorticoid hormone (GCH) exposure on the in vitro deletion of CD4+ CD8- and CD8+ CD4- T cells by staphylococcal enterotoxin B (SEB). APC fixation with the chemical cross-linker 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide (ECDI) inhibited their capacity to induce SEB-specific deletion of mature T lymphocytes. Deletion was not influenced by treatment with anti-CD28 antibodies, which modulate T-cell activation. However, it was augmented by IL-4, known to counteract anti-CD3- and GCH-induced thymocyte apoptosis, and was inhibited by dexamethasone (DEX). These results indicate that metabolically active APC are required for deletion of antigen-specific mature T cells and suggest that IL-4 and GCH can modulate this phenomenon in vitro. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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