Molecular characterization of the murine thymidylate kinase gene
| Autor: | P, Liang, L, Averboukh, W, Zhu, T, Haley, A B, Pardee |
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| Rok vydání: | 1995 |
| Předmět: |
Mice
Inbred BALB C DNA Complementary Base Sequence Cell Cycle Molecular Sequence Data 3T3 Cells Sequence Analysis DNA Simian virus 40 Thymidylate Synthase Thymidine Kinase Gene Expression Regulation Enzymologic Mice Genes Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular Growth Substances Nucleoside-Phosphate Kinase Kirsten murine sarcoma virus Cell Line Transformed |
| Zdroj: | Cell growthdifferentiation : the molecular biology journal of the American Association for Cancer Research. 6(10) |
| ISSN: | 1044-9523 |
| Popis: | Thymidylate kinase (TMK) catalyzes an essential reaction of converting dTMP to dTDP, leading to formation of the DNA precursor dTTP. Unlike the preceding enzymes, thymidine kinase and thymdylate synthase, little is known about regulation of TMK after mammalian cells exit from quiescence and enter the cell cycle. In this study, cDNA of murine TMK was isolated and characterized. Murine TMK gene expression in nontransformed BALB/c 3T3 cells was shown to be regulated during Gø to S-phase transit at both mRNA and enzyme activity levels. Its timing was distinctive from that of thymidine kinase and thymdylate synthase. In contrast, the regulation of TMK in response to serum growth factor stimulation was abolished in cells transformed by either SV40 or Kirsten viruses, and tmk expression became constitutively elevated. This finding is in concordance with previous results showing that transformed cells exhibit more relaxed control than normal cells in their initiation of DNA replication. |
| Databáze: | OpenAIRE |
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